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1.
Zootaxa ; 5397(3): 435-443, 2024 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-38221193

RESUMO

A new species of Scirtothrips is described from India, S. donumdei, collected on leaves of Senegalia pennata (Fabaceae). Partial mitochondrial cytochrome c oxidase subunit 1 (mtCOI) gene sequence of the species was sequenced and the annotated sequence was submitted to NCBI GenBank.


Assuntos
Fabaceae , Tisanópteros , Animais , Tisanópteros/genética , Índia , Folhas de Planta , Mitocôndrias
2.
Mol Biol Rep ; 50(2): 1221-1230, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36436078

RESUMO

BACKGROUND: Maconellicoccus hirsutus is a destructive pest which causes severe losses of agricultural and horticultural crops. For the management of M. hirsutus, many insecticides have been used and it has been exposed to insufficient dosage or uneven spray coverage which resulted in the development of insecticide resistance. Xenobiotic metabolism can be better understood with the help of gene expression studies by unveiling the underlying molecular mechanisms. The qRT-PCR is the simplest method to analyse gene expression, however, it highly relies on suitable reference genes concerning the different experimental conditions. METHODS AND RESULTS: We evaluated the stability of five reference genes in two sets of experimental conditions viz. developmental stages (nymphs and adults) and agrochemical stress (GA3 and Buprofezin sprayed) against M. hirsutus, using different softwares-NormFinder, geNorm, BestKeeper, and RefFinder. The study revealed that ATP51a and GAPDH can be used as reference genes for gene expression studies when exposed to Gibberellic acid. Additionally, the study revealed that the ideal pair of reference genes for data validation in M. hirsutus treated with Buprofezin was GAPDH and ß-tubulin. The ideal reference gene combination for various developmental stages was found to be 28S and Actin. CONCLUSION: According to the study, GAPDH can be utilized as a reliable reference gene in the agrochemical (GA3 and Buprofezin) exposure set. The genes can be utilized as a suitable reference for qRT-PCR gene expression studies of xenobiotic metabolism to understand the underlying molecular mechanism, which will help further to design suitable management strategies.


Assuntos
Hemípteros , Tiadiazinas , Animais , Hemípteros/genética , Xenobióticos , Reação em Cadeia da Polimerase em Tempo Real , Perfilação da Expressão Gênica/métodos , Padrões de Referência
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